Figure 6
From: TRAF4 promotes lung cancer aggressiveness by modulating tumor microenvironment in normal fibroblasts
CM from TRAF4-knockdown fibroblasts suppressed in vivo tumor progression of NSCLC in a mouse xenograft model. (A) Schematic description for generation of mouse xenograft model and treatment of CM. Radiation exposure was locally treated to xenografted tumor sites and each CM was treated for every 4 d, or rICAM1 three times weekly. (B) Effects of CM on tumor growth were measured in a mouse xenograft model. Error bars, ± SEM (n = 3); * p < 0.05 compared with irradiated tumor volume. (C) In vivo effects of CM on EMT marker of tumor were assessed by Western blotting. (D) A schematic diagram illustrates how radiation-induced TRAF4 in fibroblasts promotes lung cancer progression. TRAF4 stabilizes NOX complex and increases secretion of sICAM1 which induces EMT and radioresistance of lung cancer cells.
