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Figure 1

From: Human Primary Bone Marrow Mesenchymal Stromal Cells and Their in vitro Progenies Display Distinct Transcriptional Profile Signatures

Figure 1

FACS gating strategy and experimental design of the microarray analysis. a. Freshly isolated, lineage-depleted bone marrow mononuclear cells were stained with antibodies against CD45, CD31, CD71, CD235a and CD271 as described. Following forward/side scatter gating and dead cell exclusion, CD45−/CD31−/CD71−/CD235a− cells were sorted by gating on the CD271+ population. A representative set of FACS plots is presented. b. Schematic overview of the experimental workflow. From each donor (n = 4), primary cells were sorted into lysis buffer for gene expression analysis, and for culture in mesensphere and standard MSC medium, respectively. Ex-vivo cultured adherent BM-MSCs and mesenspheres were harvested in passages 0 and 3, and prepared for microarray analysis.

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