Figure 2

Gene expression and cluster analysis of primary, adherent and non-adherent culture-expanded BM-MSCs. (a) Heatmap of significantly differentially expressed genes in primary, adherent and sphere cultured-derived lin−/CD45−/CD31−/CD71−/CD235a−/CD271+ cells at passages 0 and 3. (b) Cluster analysis of differentially expressed genes. The first data points from the left in each plot (1–3, light blue) represent primary cells, the next 8 boxes in orange represent adherent cultured cells (4–7: passage 0; 8–11: passage 3) and the last 8 boxes in dark blue represent the results for sphere cultured cells (12–15: passage 0, 16–19: passage 3).