Figure 2

PEDF−/− steady state BM had normal hematopoiesis. Flow cytometric analysis of lineage distribution in PB (A) and BM (B) of steady state mice. Representative FACS plots of stem cell analysis (C) and the graph represents frequencies of stem and progenitor cells in BM of steady state mice (D), HSCs (LSKCD150⁺CD48⁻CD9⁺, LSKCD150⁺CD48⁻), MPPs (LSKCD150⁻CD48⁻) and progenitor cells (LSKCD150⁻CD48⁺, LSK). Number of cells per femur (E) and colony count for colony forming unit granulocyte-monocyte, CFU-GM (F) and burst forming unit-erythroid, BFU-E (G) from BM cells plated in methylcellulose media. Data are mean ± SEM, n = 4.