Figure 3

PEDF−/− BM cells give rise to stable reconstitution and normal hematopoiesis in competitive transplantation experiments. (A) Engraftment in primary recipients measured by flow cytometric analysis of engrafted CD45.2⁺ donor cells in recipient PB at four weeks. (B) Lineage distribution of CD45.2⁺ cells in PB at four weeks time point, myeloid cells: Mac/Gr, B-cells: B220 and T-cells: CD3. (C) Engraftment in BM of primary transplanted recipients 20 weeks post transplantation. (D) Lineage distribution 20 weeks post transplantation in BM of primary transplanted mice. (E) Flow cytometric analysis of LSKCD48⁻CD150⁺ cell and LSK cell frequencies in BM of primary recipients. Data are mean or shows individual mice (C and E) from three independent experiments ± SEM, n = 3 donors per genotype and 2–3 recipients per donor.