Figure 3
Distribution of unfolding lengths (ΔL, in nm) and maximal forces obtained from WLC fitting of experimental data. (a) Results from fitting a total of 2,804 unfolding by two Gaussian functions with respective mean values of ΔL = 9.5 ± 3.3 nm and 17.4 ± 3.3 nm (corresponding to IgC2 domains) and 30.6 ± 3.7 nm (FnIII domains). The inset displays the histogram obtained from 21 most representative unfolding curves (116 peaks), which indicates similar results: ΔL1: 11.0 ± 1.6 nm, ΔL2 ≈ 17.2 ± 1.8 nm and ΔL3: 30.4 ± 1.8 nm. (b) The distribution of maximal forces (Fmax, in pN) obtained from WLC model. Data for IgC2 domains (red) are fit by three Gaussian distributions, with mean values: 61.0 ± 12.9 and 95.0 ± 26.4 pN; and those for FnIII, by two Gaussians with means 79.4 ± 21.5 and 145.0 ± 48.6 pN. (c) Example of force-extension curves with WLC fitting.
