Figure 2
RT-qPCR analysis of ER stress and Atf6α target genes expression in cells treated with sodium salicylate. Wild-type and Atf6α knockout MEFs were treated with 20 mM NaSal or 3 µg/mL tunicamycin for the indicated time. Total RNA was extracted and RT-qPCR analysis of relative mRNA content for BiP/Grp78 (a), CHOP/Gadd153 (b), Pdia4 (c), Herpud-1 (d), p58ipk (e), Hrd-1 (f), total Xbp-1 (g) and spliced Xbp-1 (h) were performed after cDNA synthesis. *, ** or *** indicate statistical significant differences between groups (p < 0.05; p < 0.01 or p < 0.001, respectively) as determined by ANOVA followed by Bonferroni’s test. ns indicates not significant differences. Data were collected from three independent experiments. Results are plotted as mean ± SD.
