Figure 5
The effect of celecoxib treatment on the tumor delivery of micelles. (A) In vivo imaging of A549 xenograft-bearing mice (left) and ex vivo imaging of tumor xenografts (right) treated with or without celecoxib 24 h post-DiR-labeled micelles administration. (B) Relative fluorescence intensity per gram tissue in the homogenate of tumor xenografts (n = 4). (C) The fluorescence intensity of coumarin-6-labeled micelles per field in the tumor slices treated with or without celecoxib (n = 4). (D) In vivo distribution and penetration profiles of coumarin-6-labeled micelles in tumor tissues treated with or without celecoxib displayed by immunofluorescence staining. Right panels show the fluorescence intensity profiles of micelles from the nearest blood vessels to the tumor tissue in the selected regions of interest (ROI, indicated by dashed yellow rectangles). The dosage regimen of celecoxib was 200 mg/kg/day by gavage once a day for 14 days. **P < 0.01 compared with the control group. Blue: nuclei. Green: coumarin-6-labeled micelles. Red: CD31-labeled tumor vessels. The bar indicates 100 μm.
