Figure 2 | Scientific Reports

Figure 2

From: DrwH, a novel WHy domain-containing hydrophobic LEA5C protein from Deinococcus radiodurans, protects enzymatic activity under oxidative stress

Figure 2

Transcriptional analysis of drwH in D. radiodurans. (A) Relative expression level of drwH in response to various stresses in D. radiodurans. Total RNA of D. radiodurans was extracted after exposure to 50 mM H2O2 (30 min), 0.3 M NaCl (2 h), 50 °C (2 h), 15 °C (2 h), and 0.5 M D-sorbitol (2 h). Different letters indicate significant differences (P < 0.05). (B) drwH transcription in D. radiodurans WT and ΔirrE mutant strains under oxidative and salt conditions. Error bars represent the standard error of the mean of 3 independent experiments. The asterisk indicates a significant difference, which was calculated with Student’s t-test (*P < 0.05, NS: no significant difference). (C) Nucleotide sequence of the putative promoter region of drwH. The open boxes represent the putative σ70-dependent promoter. The start and termination codons of the drwH gene are indicated in red bold letters. The 300-bp region containing a predicted WHy domain is highlighted in blue.

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