Figure 4 | Scientific Reports

Figure 4

From: Prerequisites for the acquisition of mammalian pathogenicity by influenza A virus with a prototypic avian PB2 gene

Figure 4The alt text for this image may have been generated using AI.

Comparison of the growth rate of each mutant virus against control virus in mixed cultures. Mixtures (1:1) of wild-type rPB2(01310) and rPB2(01310)-MVV (top) or rPB2(01310) and rPB2(01310)-E627K (bottom) were inoculated in ECEs (a), EDEs (b), and MDCK cells (c). At 3 dpi, the allantoic fluid of the embryonated eggs and the cell culture supernatants of the MDCK cells were collected, and the PB2 gene was amplified by RT-PCR and sequenced. The codon for 66I is ATA, 109I is ATC, 133I is ATT, and 627E is GAG; for the PB2 variants, the codon for 66M is ATG, 109V is GTC, 133V is GTT, and 627K is AAG. The DNA sequence chromatograms shown correspond to one sample from each host.

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