Figure 5

Locations of residues 66, 109, and 133 near the PB1-PB2 and PA-PB2 interfaces in the polymerase complex model. The crystal structure of the A/little yellow-shoulder bat/Guatemala/060/2010 (H17N10) polymerase complex (PDB code: 4WSB) was used to locate amino acid residues in IAV. The analysis was performed using the program PYMOL (W. L. Delane; http://www.pymol.org). (a) The locations of the 66,109, and 133 residues in the polymerase complex. The interface of PB1-PB2 with the residues 109V (b) and 66M (d). The interface of PA-PB2 with the 133V (c) residue. Each subunit of the polymerase complex is shown in a different colour.