Figure 4
MSA down-regulates integrin β3 and inhibits phosphorylation of AKT. (A) Relative mRNA expressions of integrin subunits (αV, α1, α5, β1, β3, β5) in HUVECs exposed to 2 μM MSA for different hours (0, 3, 6, 12 h) were quantified by qPCR assay. A 2−ΔΔCT relative quantification method was employed and β-actin was used as housekeeping gene. The error bars represented the SD (n = 9) and were determined by one way ANOVA comparison test. *p < 0.05, **p < 0.01. Western blot was employed to analyze the protein levels of integrin αv, β1, β3 and AKT phosphorylation in (B) a time-dependent (2 μM, 0, 3, 6, 12, 24 h) or (C) a dose dependent (0, 1, 2 μM, 24 h) manner of MSA, and β-actin was served as loading control. The relative levels of protein were quantified by ImageJ software.
