Figure 1 | Scientific Reports

Figure 1

From: A miR-SNP biomarker linked to an increased lung cancer survival by miRNA-mediated down-regulation of FZD4 expression and Wnt signaling

Figure 1

Effects of the variant allele rs713065(C) on FZD4 mRNA and protein expression. (A) The C allele significantly down-regulates expression of a reporter consisting of the coding sequence of GFP and part of the 3′UTR of FZD4 with variant (C) and wildtype (T) alleles at rs713065. Data are from six independent transfections. (B) Down-regulation of reporter gene expression by the rs713065(C) allele in H1299, H226, A549, H661, and H1975 cells. Data are from six independent transfections. (C) Dual luciferase reporter with the rs713065 variant allele. Firefly luciferase activity is normalized to sea pansy luciferase activity. The presence of the C allele results in the down-regulation of luciferase activity. Shown are the results of triplicate measurements (mean ± SE). (D) rs713065 (C) down-regulates FZD4 mRNA expression as determined by qRT-PCR (mean ± SE) (n = 3). *p < 0.05; **p < 0.001. (E) rs713065(C) down-regulates FZD4 protein expression, as determined by Western blot with FLAG-labelled protein. Full length Western-blot images with different intensities were shown in supplementary Fig. S1.

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