Figure 4 | Scientific Reports

Figure 4

From: A miR-SNP biomarker linked to an increased lung cancer survival by miRNA-mediated down-regulation of FZD4 expression and Wnt signaling

Figure 4

Inhibition of tumor colony formation and cell migration by FZD4-miR-SNP in NSCLC cells. (A) The rs713065(C) allele reduces tumor cell induced-clonogenesis in C/C homozygous H1299 cells as well as C/T heterozygous Calu6 cells transfected with the FZD4-3′UTR-SNP plasmid, compared with those transfected with the FZD4-3′UTR-WT expression plasmid. *p < 0.05; **p < 0.001. (B) FZD4-3′UTR-SNP inhibits H1299 cell mobility compared with mobility in cells transfected with the FZD4-3′UTR-WT plasmid. Wound healing of cultured H1299 cells using phase contrast. Photomicrographs were taken at 0 h and 36 h. (C) Inhibition of tumor cell migration/invasion in H1299 cells transfected with FZD4-3′UTR-SNP or FZD4-3′UTR-WT plasmids by a FluoroBlock membrane assay in transwell Boyden chambers.

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