Figure 3

CP extract suppresses pancreatic β cell apoptosis in diabetic mice. The type 2 diabetic mice were induced by feeding with high-fat diet for 4 weeks and then injecting intraperitoneally with 25 mg/kg STZ for 3 days consecutively. The diabetic mice with consecutive 7-day hyperglycemia (11 mmol/L or greater) were selected for the experiment and then CP extract or glibenclamide were administered to mice for consecutive 5 weeks. At the end of experiment, mice were sacrificed. The samples of pancreas tissues were collected. (A) TUNEL staining of mice pancreas tissues (magnification, 600×) and (C) Immunofluorescence analysis of cleaved caspase-3 expression in mice pancreas tissues (magnification, 600×); (B) The number of TUNEL-positive cells; (D) Cleaved caspase-3-positive area. All data are presented as means ± SEM (n = 8). ^#p < 0.05, ^##p < 0.01 and ^###p < 0.001, diabetic model group compared with non-diabetic groups; ^*p < 0.05, ^**p < 0.01 and ^***p < 0.001, compared with diabetic model group. CON: non-diabetic control group; CON-CP: CP extract-treated non-diabetic control group; MOD: diabetic model group; GLB: glibenclamide-treated diabetic group.