Figure 5

CP extract suppresses MAPK signals and activates Akt signal in pancrease tissues of diabetic mice. The type 2 diabetic mice were induced by feeding with high-fat diet for 4 weeks and then injecting intraperitoneally with 25 mg/kg STZ for 3 days consecutively. The diabetic mice with consecutive 7-day hyperglycemia (11 mmol/L or greater) were selected for the experiment and then CP extract or glibenclamide were administered to mice for consecutive 5 weeks. At the end of experiment, mice were sacrificed. The samples of pancrease tissues were collected. Protein expressions of p-p38, p38, p-ERK, ERK, p-JNK, JNK, p-Akt and Akt were determined in pancreas tissues using Western blot. (A) Representative image of Western blot; (B) Protein expressions of p-p38, p-ERK, p-JNK and p-Akt in pancreas tissues. (C) Protein expressions of p38, ERK, JNK, and Akt in pancreas tissues. All data are presented as means ± SEM (n = 8). ^#p < 0.05, ^##p < 0.01 and ^###p < 0.001, diabetic model group compared with non-diabetic groups; ^*p < 0.05, ^**p < 0.01 and ^***p < 0.001, compared with diabetic model group. CON: non-diabetic control group; CON-CP: CP extract-treated non-diabetic control group; MOD: diabetic model group; GLB: glibenclamide-treated diabetic group.