Figure 3 | Scientific Reports

Figure 3

From: A Systematic Protein Refolding Screen Method using the DGR Approach Reveals that Time and Secondary TSA are Essential Variables

Figure 3

DGR of IL-17A. (a) Melting transition curves of IL-17A in CHC buffer at pH 9–10 in the presence or absence of arginine. (b) Melting transition curves of IL-17A in single buffer at pH 9–10 in the presence or absence of arginine. (c) Melting transition curves collected at different time points in CHES pH 9.5, 0.4 M Arg. (d) Melting transition curves in CHES pH 9.5 in the presence of redox agents. (e) Results of the TSA buffer screen. (f) Characterization of refolded IL-17A by SLS, demonstrating presence of dimer. (g) Crystal of IL-17A.

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