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Figure 1

From: 2,4-D attenuates salinity-induced toxicity by mediating anatomical changes, antioxidant capacity and cation transporters in the roots of rice cultivars

Figure 1

Histochemical detection of lipid peroxidation by Schiff’s reagent in roots of rice cultivars under light microscope (A) ZJ 88 and (B) XS 134. Bright fluorescence at root tips shows accumulation of aldehydes. Histochemical detection of (C,D) H2O2 and (E and F) O2 − by DAB and NBT in rice cultivars ZJ 88 (C,E) and XS 134 (D,F) roots, respectively. Brown color shows H2O2 accumulation, while blue coloration represents O2 − production. 20-day-old rice seedlings were treated as control (CK) with EC 1.2 dS m−1), T2 (recommended dose of 2,4-D), T3 (EC 4 dS m−1), T4 (EC 8 dS m−1), T5 (EC 4 dS m−1 + recommended dose of 2,4-D), T6 (EC 8 dS m−1 + recommended dose of 2,4-D) for 15 days Afterwards, the roots were stained with Schiff’s reagent, 3,3′-diaminobenzidine (DAB) or nitroblue tetrazolium (NBT), respectively, and immediately photographed under a light microscope (LEICA MZ 95 microscope equipped with LECIA DFC 300 FX camera). The experiment was repeated four times with similar results. Scale bar = 2 mm.

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