Figure 4
Peripheral M6a glycoprotein levels are affected by stress. (A,B) ELISA determination of M6a levels in serum samples of a control C57Bl/6 J mouse population. Both male (A) and female (B) samples show a normal distribution. Female mice n = 10; Shapiro-Wilk normality test P = 0.3; male mice n = 24; P = 0.6. (C-F) Female and male mice were subjected to a 3-week protocol of chronic restraint stress (CRS, black boxes). CRS consisted of 5 days of daily restraint stress followed by a 2-day interruption. Mice were sacrificed at the end of week 3. M6a levels in hippocampal homogenates (C, D) and in serum (E,F) were determined by ELISA. n = 9–10 for females and n = 6–8 for males. *P < 0.05. (G–J) To evaluate stress effect on M6a levels in individual male and female mice three blood extraction procedures were performed (arrowheads between boxes), the serum from the first and third extractions were assayed for M6a levels. (G, H) effect of blood extraction alone (white boxes) (I, J) blood extraction plus CRS (black boxes). Each line represents one individual. Gray line represents mean values. T-student test followed by Dunnet multiple comparison test *P < 0.05. n = 6 for males and n = 5 for females.
