Figure 7
From: The role of the immunoproteasome in interferon-γ-mediated microglial activation
Immunoproteasome inhibition alters phagocytosis. (a) BV-2 cells were treated with IFNγ and/or ONX-0914 for 24 h. Treatment significantly alters phagocytosis of fluorescent microspheres (F(3,20) = 26.91, p > 0.001, n = 6, ANOVA with Tukey’s post hoc). Both IFNγ and ONX treatments significantly decrease phagocytosis (p = 0.001; p < 0.001, respectively). Co-treatment of IFNγ and ONX further decreases phagocytosis compared to control (p > 0.001), IFNγ (p = 0.001) and ONX (p = 0.008). (b) Phagocytosis in β5i KO BV-2 cells is different between groups (F(3,20) = 14.7, p < 0.001, n = 6). Mean fluorescence intensity is higher in control cells compared to IFNγ and ONX-0914/IFNγ co-treatment (p = 0.001 and p < 0.001, respectively). There was no difference between control and ONX-0914 treated cells.
