Figure 2 | Scientific Reports

Figure 2

From: Miltefosine Resistant Field Isolate From Indian Kala-Azar Patient Shows Similar Phenotype in Experimental Infection

Figure 2

Generation of Nitrite production and ROS in the studied isolates (AG83, T8, T9) infected and MIL treated macrophages. Culture supernatant was used to evaluate (a) NO generation by Griess method and (b) ROS generation was measured by H2DCFDA probe. Data represents mean ± SD of three independent experiments; *P < 0.05,**P < 0.001, ***P < 0.0001(Student’s t-test), representing significant differences between studied isolate infected macrophages and MIL-treated infected macrophages in (a) NO and (b) ROS production respectively. NS: Non significance.

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