Figure 3

Assessment of mitochondrial transmembrane potential in different clinical isolates (AG83, T8, T9) and viable Leishmania isolates following MIL treatment. (a) Mitochondrial membrane potential (ΔΨm) in studied isolates following MIL treatment were measured using 5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazole carbocyanide iodide (JC-1) probe and (b) the bar graph showing the ratio of fluorescence (590 nm/530 nm) measured by fluorescence activated cell sorting analysis. (c) Annexin V fluorescein isothiocyanate (FITC)- Propidium Iodide(PI) Assay in the studied isolates following MIL treatment were performed to assess viable Leishmania isolates and the lower left quadrant of each dot plot represents viable isolates. (d) The bar graph showing the viable cells as determined by fluorescence activated cell sorting analysis. Data are expressed as the mean ± SD of three independent experiments and levels of significance are indicated by P values.