Figure 5

Study of Organomegaly and Estimation of parasite burden in the spleen and liver of the experimental groups of animals. Hamsters were infected with studied parasites and infection was allowed to establish for next 8 weeks. 8 weeks infected hamsters received MIL doses. After 45 days post treatment hamsters were sacrificed and (a) Liver weight and (b) Spleen weight of the hamsters of different experimental groups (AG83-INF, AG83-TRE; T8-INF, T8-TRE; T9-INF, T9-TRE) were determined. Data represents mean ± SD of 5 animals per group; unpaired two-tailed Student’s t-test was performed and levels of significance are indicated by P values. On the other hand, after 45 days post treatment hamsters were sacrificed and the (c) hepatic as well as the (d) splenic parasite load was determined by stamps-smear method. Total parasite load in each organ is expressed in LDU unit. 1 LDU = amastigote per nucleated cell x organ weight in milligram. Data represents mean ± SD of 5animals per group; unpaired two-tailed Student’s t-test was performed and levels of significance are indicated by P values.