Figure 4

CIA induced necroptosis. (A) Joint tissues from normal and CIA mice were stained with haematoxylin and eosin, and Safranin O (n = 6). (B) Immunohistochemical visualization of RIPK1, RIPK3, and MLKL in the synovium of normal and CIA mice (n = 4). (C) Joint tissues from normal and WT and IFN-γ-knockout CIA mice were stained with haematoxylin and eosin, and Safranin O (n = 6). (D) Immunohistochemical visualization of RIPK1, RIPK3, and MLKL in the synovium of WT and IFN-γ-knockout CIA mice (n = 4). Data are expressed as mean ± SD (***P < 0.005).