Figure 1
From: Intracellular Chloride Regulation in AVP+ and VIP+ Neurons of the Suprachiasmatic Nucleus
RCl measurement in genetically-identified SCN neurons. Confocal micrographs demonstrating regional expression of Cl-Sensor in the SCN of AVP::Cl-Sensor (A) and VIP::Cl-Sensor (B) mice. Native Cl-Sensor fluorescence is depicted in green, and DAPI stain is shown in blue. Pseudocolored epifluorescent micrographs of an AVP::Cl-Sensor SCN slice exhibiting YFP (C) and CFP (D) emission. (E) Baseline RCl was higher during the day (ZT 2 to 8) compared to the night (ZT 12 to 18) for both AVP+ (GEE, p < 0.05) and VIP+ (GEE, p < 0.001) neurons.
