Figure 3
From: Intracellular Chloride Regulation in AVP+ and VIP+ Neurons of the Suprachiasmatic Nucleus
The KCCs regulate [Cl−]i in SCN neurons. (A) Example experiment from an AVP::Cl-Sensor mouse recorded during the night demonstrating the effect of 10 µM of the KCC antagonist VU. VU caused an increase in RCl indicative of a rise in [Cl−]i. Each trace represents a RCl measurement obtained from a single neuronal soma. (B) Example experiment from a VIP::Cl-Sensor mouse recorded during the day demonstrating the effect of VU. VU caused an increase in RCl indicative of a rise in [Cl−]i. (C) Summary data of the average change in RCl after VU by neuron type and time of day. VU resulted in an increase in RCl in all conditions (GEE, p < 0.005), but had a significantly greater effect in VIP+ neurons compared to AVP+ neurons (GEE, p < 0.05). (D) Summary of changes in RCl elicited by VU in a HEPES-buffered solution. VU had a larger effect during the day in VIP+ neurons when compared to VIP+ night (GEE, p < 0.05) and AVP+ day neurons (GEE, p < 0.001). For (C) and (D), the number of slices and total regions of interest (in parentheses) is listed for each condition under the x-axis.
