Figure 4
From: Intracellular Chloride Regulation in AVP+ and VIP+ Neurons of the Suprachiasmatic Nucleus
NKCC1 contributes to setting resting [Cl−]i in SCN neurons. (A) Example experiment from an AVP::Cl-Sensor mouse recorded during the night showing the effect of blocking NKCC1 with 10 µM of bumetanide. Bumetanide caused a small increase in RCl. Each trace represents a RCl measurement obtained from a single neuronal soma. (B) Example experiment from a VIP::Cl-Sensor mouse recorded during the night demonstrating the effect of bumetanide. Bumetanide caused a small decrease in RCl. (C) Summary data of the average change in RCl after bumetanide by neuron type and time of day. Bumetanide elicited small but statistically significant changes in RCl in each condition (GEE, p < 0.005). AVP+ and VIP+ neurons responded differently to bumetanide (GEE, p < 0.001), but there were no day/night differences within neuron types. Bumetanide had a statistically different effect on VIP+ neurons compared to AVP+ neurons. (D) Summary of changes in RCl elicited by bumetanide in a HEPES-buffered solution. Bumetanide had a larger effect in AVP+ neurons compared to VIP+ neurons (GEE, p < 0.001). For (C) and (D), the number of slices and total regions of interest (in parentheses) is listed for each condition under the x-axis.
