Table 5 Discrepant results between the BYG Carba v2.0 test and the reference method.

From: Multicentre evaluation of the BYG Carba v2.0 test, a simplified electrochemical assay for the rapid laboratory detection of carbapenemase-producing Enterobacteriaceae

Species

Carbapenemase

First testing

Confirmation in centre A

Additional hydrolysis testing

BYG value at 30 min (AU)

Result

Time to pos (min)

BYG value at 30 min (AU) repeat

Time to pos (min) repeat

Result repeat

Carba NP test

1

Enterobacter cloacae

GES-5

0.4

FN

4.6

FN

POS

2

Enterobacter cloacae

GES-5

2.9

FN

−4.3

FN

POS

3

Klebsiella oxytoca

GES-5

−1.3

FN

-

0.8

FN

NEG

4

Klebsiella oxytoca

GES-5

−0.5

FN

12.3

28

TP

POS

5

Enterobacter cloacae

IMI-1

−5.0

FN

94.0

6

TP

POS

6

Enterobacter cloacae

IMI-2

11.0

FN

0.4

FN

NEG

7

Escherichia coli

IMP-1

2.0

FN

−0.2

FN

POS

8

Enterobacter cloacae

IMP-1

6.1

FN

12.1

27

TP

NEG

9

Providencia stuartii

NDM-1

1.9

FN

−1.6

FN

NEG

10

Providencia rettgeri

NDM-1

9.3

FN

11.4

FN

NEG

11

Klebsiella oxytoca

NDM-1 *

8.4

FN

0.9

TN

NEG

12

Proteus mirabilis

NDM-1

3.9

FN

14.9

24

TP

POS

13

Klebsiella pneumoniae

OXA-181

3.1

FN

3.5

FN

NEG

14

Escherichia coli

OXA-244

0.9

FN

2.2

FN

NEG

15

Escherichia coli

OXA-244

1.2

FN

3.1

FN

NEG

16

Escherichia coli

OXA-244

3.7

FN

16.3

24

TP

POS

17

Escherichia coli

OXA-48

9.4

FN

8.8

FN

POS

18

Enterobacter cloacae

VIM-1

6.8

FN

6.0

FN

NEG

19

Providencia stuartii

NDM

−0.1

FN

32.5

16

TP

NEG

20

Klebsiella oxytoca

OXA-232

−4.1

FN

1.2

FN

POS

21

Escherichia coli

OXA-244

1.1

FN

0.8

FN

POS

22

Escherichia coli

OXA-48

3.6

FN

71.4

6

TP

POS

23

Escherichia coli

OXA-48 *

0.2

FN

−1.1

TN

NEG

24

Klebsiella pneumoniae

OXA-48

10.3

FN

10.0

FN

NEG

25

Enterobacter cloacae

VIM-1

8.0

FN

9.1

FN

POS

26

Escherichia coli

VIM-4

1.1

FN

8.2

FN

POS

27

Enterobacter cloacae

No Carba (SHV-12)

15.8

FP

20

1.1 and 4.1

TN

NEG

  1. Isolates 1 to 18 were retrospectively collected and the isolates 19 to 27 are prospectively collected.
  2. The Carba NP imipenem hydrolysis test was performed to confirm the imipenem hydrolysis in these isolates.
  3. FN: False negative; TP: True positive; TN: True negative; POS: positive; NEG: negative.
  4. *These isolates have probably lost their plasmid.
  5. Correct results obtained in centre A are in bold and underlined.