Figure 4
From: In-line balanced detection stimulated Raman scattering microscopy

Comparison between unbalanced and IBD-SRS imaging. (a–d) 120 × 120 pixels images of a 40 × 40 μm2 sample area of a blend of 6-μm PMMA and 3-μm PS beads acquired at the PMMA Raman resonance of 2953 cm−1. (a,c) IBD-SRS images at integration times of 50-μs and 500-μs, respectively. (b,d) as above, but under unbalanced SRS as a sake of comparison. (e,f) SRS spectra of PMMA at the point X indicated in the panels above, under IBD- (blue) and unbalanced-SRS (red) at integration times of 50-μs and 500-μs, respectively.