Figure 4
From: Orchestrated Domain Movement in Catalysis by Cytochrome P450 Reductase
Effects of salt concentration on the kinetics of ET by CPR and on its conformation. (a) Stopped-flow traces showing the reduction of cytochrome c on rapid mixing of CPR pre-reduced with NADPH and cytochrome c, at various concentrations of added salt; the percentage of a single turnover which occurred within the 2ms deadtime of the instrument is plotted as a function of salt in (b). (c) Pairwise distance distribution functions derived from the SANS data at different salt concentrations, showing that increasing the salt concentration leads to a more extended conformation, in qualitative agreement with SAXS studies37, 45. (d) Porod-Debye plots, showing increased flexibility of CPR at ≥0.6 M NaCl.
