Figure 2
From: Mapping the key residues of SufB and SufD essential for biosynthesis of iron-sulfur clusters
Functional residues of SufB and SufD in the SufBCD complex. (a) Distinct distributions of the functional residues depicted in the crystal structure of the SufBCD complex (PDB code: 5AWF). Region A is located at the N-terminal part of the β-helix core domain of SufB and contains six critical residues of SufB (SufBR226, SufBN228, SufBC254, SufBQ285, SufBW287, and SufBK303; depicted in pink), among which SufBC254 was found to be essential for the sulfur-transfer from SufE. Temperature-sensitive mutations are also located in this region (SufBY224, SufBQ234, SufBE236, and SufBE252; yellow). Region B is located at the interface between SufB and SufD and contains three critical residues (SufBC405, SufBE434, and SufDH360; pink). In addition, SufBE432, SufBH433 and SufDC358 (green) residing in region B appear to share a redundant role. (b) Surface representation of the SufBCD complex. The SufBC254 residue is located in a pit on the N-terminal side of the β-helix core domain of SufB. The side chain is shown in yellow.
