Figure 6

3D structure of a frozen-hydrated marine cyanobacterium, capturing the penetration of a cyanophage into the cell membrane. (a,b) 5.4-nm-thick slices of the cell in the XY plane reconstructed by GENFIRE and FBP, respectively, where the Y axis is the tilt axis and the beam direction is along the Z axis. Magnified views of the penetration of a cyanophage for the GENFIRE and FBP reconstructions in the XY (c,d), XZ (e,f), and ZY (g,h) planes, respectively. The top side of the membrane is visible in both reconstructions (magenta arrows), but the bottom side is only visible with GENFIRE (yellow arrow). (i,j) Isosurface renderings of the penetration of the cyanophage to the cell membrane. Overall, GENFIRE exhibits higher contrast, less peripheral noise, more easily detectable cell boundaries than FBP.