Figure 3
From: A broad range quorum sensing inhibitor working through sRNA inhibition
Changes in expression of rnaIII and spa by ajoene. Expression measured by reporter fusion based plate assay (a,b), Northern blot (c,d) and qRT-PCR (e,f). (a) Expression of rnaIII measured with rnaIII::lacZ reporter fusion. (b) Expression of spa measured with spa::lacZ reporter fusion. (c) Expression of rnaIII and (d) expression of spa with the addition of 1 μg/ml and 5 μg/ml of ajoene compared to controls with addition of DMSO or H2O. The right panels in (c,d) show the loading controls of ethidium bromide stained total RNA. (e) qRT-PCR data of samples retrieved at exponential growth phase and, (f) at stationary growth phase for rnaIII (dark grey bars), spa (medium grey bars) and hla (light grey bars).
