Figure 6

Effects of melatonin (M) and restraint stress on Nrf2/HO-1 signaling pathway. (A) The expression of Nrf2 and HO-1 in testes of different groups of mice was detected by Western blot (n = 5). (B,C) The relative expression level of Nrf2 and HO-1 normalized to ACTB (n = 5). All data are presented as mean ± SEM. **P < 0.01 vs. control group; ***P < 0.001 vs. control group; ##P < 0.01 vs. stress group. Control, non-stress treated with saline; Control+M, non-stress treated with melatonin; Stress, stress treated with saline; Stress+M, stress treated with melatonin.