Figure 5 | Scientific Reports

Figure 5

From: Plasmodium falciparum GPCR-like receptor SR25 mediates extracellular K+ sensing coupled to Ca2+ signaling and stress survival

Figure 5

Wt parasites were more resistant to starvation than PfSR25 − parasites. (A) Parasites were cultured in albumax-free media for 3 days. Albumax was reintroduced into albumax-free cultures on day 3. Parasitemia was determined by DHE staining after 0 and 6 days. (B) Histogram shows representative FACS dot plot showing the fluorescence intensities in Fig. 5A. (C) Parasites were cultured in albumax-free media for 3 days. Albumax was reintroduced into albumax-free cultures on day 3. Parasites were cultured for 10 d, with regular medium changed. Parasites were fixed in 2% PFA and the parasitemia was determined by YOYO-1 staining. Dot-plot was obtained after FACS analysis of YOYO-1 stained parasites. FL1 and FL2: fluorescence intensity; SSC: side scatter. (D) Graph showing the parasitemia during albumax-starvation experiment performed in Fig. 5C. The graphs show the mean ± S.E.M. of three independent experiments each performed in triplicate with the controls parasites (day 0) normalized to 100%. *p < 0.05, **p < 0.01, ***p < 0.001 compared with their respective day 0 parasites.

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