Figure 6
From: Plasma biomarker proteins for detection of human growth hormone administration in athletes
Western blot analysis of two rGH plasma biomarkers. (A) = APOL1 and (B) AHSG. For 1-D Western blot analysis, all the samples were analysed however, data presented in this figure only for treatment weeks (4 and 8). For 2-D Western analysis, different subject’s samples were analysed due to limited sample availability. (A) It appeared more APOL1 isoforms and over-expressed in the lower ~37 kDa region in patients treated with rGH compared to placebo-treated patients as indicated by the arrows. (B) It appeared that AHSG has ‘train’ of isoforms ~45 kDa in the patients treated with placebo whilst two sets of isoforms (one set ~45 kDa and another set of isoforms with higher masses, boxed) were observed in patients treated with rGH only. Western blot images shown in this Figure were cropped; uncropped full length images are shown in the Supplementary Information Fig. S6.
