Figure 6
ND-PTX-Cet enhances the cellular uptake ability and apoptosis in the EGFR-expressed CRC cells. (a) The conjugation model of ND-PTX and Cet. (b) The surface charges of Cet, ND-PTX and ND-PTX-Cet were measured by Zeta potential instrument. (c) The EGFR protein levels in SW620 and HCT116 cells were analyzed by western blot. (d) The green color shows the location of EGFR proteins. Nuclei were stained with the Hoechst 33258 (blue). (e) After treatment with or without 10 μg/mL of ND-PTX or ND-PTX-Cet for 24 h, the cells were analyzed by flow cytometry. (f) The fluorescence intensity of ND-PTX in cells was quantified. The bar represents the mean ± S.E. *p < 0.05 indicates significant difference between ND-PTX and ND-PTX-Cet. (g) The cells were left treated with or without 1 μg/mL of ND-PTX or ND-PTX-Cet for 48 h. The cell morphology was observed under a live-cell imaging microscope. (h) The green color shows the location of ND particles. EGFR (red) and nuclei (blue) were exhibited.
