Figure 3

Visualization of a synchronized RP and IM event corresponding to the same particle detection. (a) Times of RP (bottom) and image (top) events after alignment. Each line corresponds to a single particle translocation. The events occur over a span of one second. (b,c) Synchronized RP and image data corresponding to the same particle translocation. The blue dashed line shows the particle’s trajectory through the channel. Gold dashed lines connect the particle’s position and the current amplitude at the same instant in time. (d) A coordinate system is defined for each channel based on four corner positions (× symbols). The channel’s origin \({\overrightarrow{x}}_{0}\), denoted by ★, is defined as the average position of the two entrance-side corners, and coordinate axes \(\hat{x}\) and \(\hat{y}\) are determined by averaging the vectors connecting the corners. The gold-colored vector \({\overrightarrow{x}}_{c}\) is the position of the particle in the channel’s coordinate frame, and is used to create the channel’s resistance map. (e) x− (blue) and y− (red) trajectories of the particle in time. The gray shaded regions indicate the times/positions that the particle was inside the channel. Notice that the particle’s x− velocity (slope of x(t)) is increased inside the channel. The red curve indicates that the particle’s y− trajectory followed the contour of the channel edge.