Figure 3

Capillaries appear unaffected in Gars ^C201R/+ skeletal muscles. (A,B) Representative single plane (A, top) and collapsed Z-stack (A, bottom and B) images of IB4 (green) staining in one month lumbrical (A) and TVA muscles (B). Scale bars = 1 mm (A, top) and 20 μm (A, bottom and B). (C,E,G) No defects in capillary diameter (C, age, P = 0.007; genotype, P = 0.090; interaction, P = 0.508), density (E, age, P < 0.001; genotype, P = 0.290; interaction, P = 0.625), or branching density (F, age, P < 0.001; genotype, P = 0.468; interaction, P = 0.721) were observed in Gars ^C201R/+ mouse lumbrical muscles at one or three months. (D,F,H) The TVA muscle also showed no difference in capillary diameter (D, age, P = 0.875; genotype, P = 0.264; interaction, P = 0.359), density (F, age, P < 0.001; genotype, P = 0.223; interaction, P = 0.889), or branching density (H, age, P = 0.020; genotype, P = 0.086; interaction, P = 0.340). All data sets were analysed with two-way ANOVAs. n = 5. See also Fig. S2.