Figure 5

The blood vessel network is unperturbed in Gars ^C201R/+ retinas. (A) Representative single plane (top) and collapsed Z-stack (bottom) images of IB4 (green) staining in one month retina. Scale bars = 1 mm (top) and 20 μm (bottom). (B–E) No defects in capillary diameter (B, age, P = 0.823; genotype, P = 0.112; interaction, P = 0.105), density (C, age, P = 0.878; genotype, P = 0.638; interaction, P = 0.504), branching density (D, age, P = 0.042; genotype, P = 0.548; interaction, P = 0.345), or major radial branch (arteries and veins, arrow in A) number (E, age, P = 0.541; genotype, P = 0.395; interaction, P = 0.188) were observed in Gars ^C201R/+ mouse retinas at one or three months. n = 5. (F) Representative single plane images of IB4 (i) and αSMA (red, ii) staining in P6-7 retina. Letters a and b (i) indicate how vascular extension was assessed (a /a + b) and the arrowhead (ii) highlights an αSMA⁺ artery. Scale bars = 1 mm. (G,H) There is no difference in IB4⁺ vascular extension (G, P = 0.818) or αSMA⁺ artery number (H, P = 0.967) between wild-type and Gars ^C201R/+ P6-7 retinas. n = 3 wild-type and 5 Gars ^C201R/+. Data sets were analysed with either a two-way ANOVA (B–E) or an unpaired, two-tailed t-test (G,H). See also Fig. S2.