Table 1 Primers used for qPCR analysis.

From: Neuropilin 1 sequestration by neuropathogenic mutant glycyl-tRNA synthetase is permissive to vascular homeostasis

Gene

Ensembl ID

Forward Primer (5′-3′)

Reverse Primer (5′-3′)

Angpt1

ENSMUSG00000022309

GCT GAC AGA TGT TGA GAC C

TGT CTG TTG GAG AAG TTG C

Angpt2

ENSMUSG00000031465

AGT CCA ACT ACA GGA TTC ACC

TGT CCG AAT CCT TTG TGC

Gapdh

ENSMUSG00000057666

TGT GTC CGT CGT GGA TCT GA58

CCT GCT TCA CCA CCT TCT TGA58

Nrp1

ENSMUSG00000025810

GGC TGT GAA GTG GAA GCA CC

AGT GGT GCC TCC TGT GAG C

Nrp2

ENSMUSG00000025969

CCG CAC GTT ACT ATT TGA TCC

TCA GAG AAG GTG GAG GAG G

Pecam1

ENSMUSG00000020717

ATT GAG GCG CAG GAC CAC G

AAG GAC TCC TGC ACG GTG ACG

Tie1

ENSMUSG00000033191

GGG AGA TAG TGA GCC TTG G

CTC GTA CAC TTC GTC ATC G

Tie2 (Tek)

ENSMUSG00000006386

CAC CGA GGC TAT TTG TAC C

CTG TAC TGT TGG CGA TGG

VEGF-A

ENSMUSG00000023951

CAT GCC AAG TGG TCC CAG G

CTC AAT CGG ACG GCA GTA GC

VEGFR1 (Flt1)

ENSMUSG00000029648

TAT GCG TGC AGA GCC AGG

GCC ACT GAT GGA GAC CTC G

sVEGFR1 (sFlt1)

ENSMUSG00000029648

TAT GCG TGC AGA GCC AGG

TCC GAG AGA AAA TGG CCT TTT59

VEGFR2 (Kdr)

ENSMUSG00000062960

TCT TTG CGC TAG GTA TCC

CTC ACA GAA GAC CAT GCC

  1. Angpt1/2, angiopoietin 1/2; Gapdh, glyceraldehyde 3-phosphate dehydrogenase; Nrp1/2, neuropilin 1/2; Pecam1, platelet endothelial cell adhesion molecule 1; Tie1/2, tyrosine kinase with immunoglobulin-like and EGF-like domains 1/2; VEGF-A, vascular endothelial growth factor A; VEGFR1/2, vascular endothelial growth factor receptor 1/2; sVEGFR1, soluble VEGFR1.
Back to article page