Figure 7
Evidence that the diminished oogenic potential in reproductively aged females is not due to an absence of OSCs in the ovaries. (a) Yield of FACS-purified OSCs from ovaries of female mice at early, mid- and late reproductive ages (mean ± s.e.m., n = 3–4 mice per group). (b,c) Quantitative analysis of Prdm1 and Stra8 promoter-driven Gfp mRNA levels in ovaries of pStra8-Gfp mice at early and late reproductive ages (mean ± s.e.m., n = 6 mice per group; *P < 0.05). (d) Yield of Stra8 promoter-driven GFP-positive cells from ovaries of pStra8-Gfp mice at early and late reproductive ages (mean ± s.e.m., n = 6–8 mice per group with 2 mice for each sample and 3–4 samples at each age). (e–g) Quantitative analysis of endogenous Stra8, Sycp3 and Nobox mRNA levels in ovaries of pStra8-Gfp mice at early and late reproductive ages (mean ± s.e.m., n = 6 mice per group; *P < 0.05).
