Table 1 NADPH oxidation rate and coupling efficiency of the CYP109B1-catalysed conversion of myristic acid, supported by different redox partners.

From: Engineering of versatile redox partner fusions that support monooxygenase activity of functionally diverse cytochrome P450s

Reconstituted systema

NADPH oxidation rateb

Couplingc efficiency (%)

Fpr / YkuN / CYP109B1 – [4:4:1]

26.0 ± 1.9

28.3 ± 3.6

YR / CYP109B1 – [4:1]

3.1 ± 0.3

49.8 ± 8.3

YR-P1 / CYP109B1 – [4:1]

12.7 ± 1.3

62.1 ± 5.1

YR-P5 / CYP109B1 – [4:1]

11.3 ± 1.9

48.9 ± 3.4

YR-G1 / CYP109B1 – [4:1]

6.6 ± 0.3

72.4 ± 3.6

YR-G5 / CYP109B1 – [4:1]

1.4 ± 0.2

81.2 ± 4.1

  1. Presented data represent average values of at least three independent reactions. The ratio of NADPH:myristic acid employed was 1:1 (200 µM each, respectively) aValues in brackets indicate applied ratio as well as final concentration (µM) of indicated proteins. bRates are given in nmol NADPH per nmol CYP109B1 per minute. The background NADPH oxidation rate in the absence of redox partner(s) was 0.1 ± 0.0. cThe coupling efficiency equals the myristic acid conversion achieved upon NADPH depletion (see also Supplementary Fig S5).
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