Figure 7 | Scientific Reports

Figure 7

From: RETRACTED ARTICLE: Activation of PERK-Nrf2 oncogenic signaling promotes Mdm2-mediated Rb degradation in persistently infected HCV culture

Figure 7

Western blot analysis shows the effect of ER-stress inducer (TG), inhibitor (TUDCA) and siRNA mediated silencing of Mdm2 on the expression of Rb in HCV infected and uninfected hepatic cells. (A) HCV infected Huh-7.5 cells were treated with variable concentrations of TG. After 24 hours, the expression of Mdm2 and Rb expression were examined by Western blot analysis. (B) Similar analysis was performed using an immortalized human hepatocyte cell line (HTERT) after treatment with TG. (C) HCV infected cells were treated with ER-stress inhibitor (TUDCA). The expression of Mdm2 and Rb levels were examined by Western blot. The expressions of PERK and Nrf2 were measured using the same lysate (D) TUDCA treatment of uninfected immortalized human hepatocytes (E) Silencing Mdm2 restores Rb expression. (F) Cyclin dependent kinase (CDK) inhibitor, roscovitine inhibits Mdm2 and restores Rb expression in HCV infected culture. (G) Pre-treatment with proteasome inhibitor (Mg132) restores Mdm2-mediated Rb degradation.

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