Figure 8 | Scientific Reports

Figure 8

From: RETRACTED ARTICLE: Activation of PERK-Nrf2 oncogenic signaling promotes Mdm2-mediated Rb degradation in persistently infected HCV culture

Figure 8

Activation of Mdm2/Rb degradation by ER-stress. HCV infected cells were treated with different concentrations of siRNAs targeted to three branches of UPR using lipofectamine. After 72 hours, cell lysates were prepared and 20 µg of proteins were loaded on to SDS-PAGE. The expressions of Mdm2 and Rb levels were examined by Western blotting. (A) Knockdown efficiency of IRE1α and its impact on the expression levels of Mdm2/Rb. (B) Knockdown efficiency of ATF6α and its impact on the expression levels of Mdm2/Rb. (C) Knockdown efficiency of PERK and its impact on the expression levels of Mdm2/Rb. The expressions of Nrf2 and pNrf2 were examined in the same lysate. (D) Western blot for Mdm2, Rb and Nrf2 activation of HCV infected cells treated with PERK inhibitor. (E) Knockdown efficiency of Nrf2 and its impact on the expression levels of Mdm2/Rb. The expression of HCV core did not change during the siRNA treatment. (F) The levels of PERK, IRE1, ATF6, Mdm2 and Nrf2 levels were not altered in the cells transfected with scrambled siRNA or GAPDH siRNA.

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