Figure 3 | Scientific Reports

Figure 3

From: Hydrogen peroxide dynamics in subcellular compartments of malaria parasites using genetically encoded redox probes

Figure 3

Dynamic range of roGFP2-Orp1 and Mito-roGFP2-Orp1 in transfected NF54-attB parasites. NF54-attB parasites transfected with roGFP2-Orp1 or Mito-roGFP2-Orp1 were exposed to 1 mM DIA or 10 mM DTT for 2 min before blocking with 2 mM NEM. Fluorescence ratios of 405/488 nm were detected with CLSM. NF54[roGFP2-Orp1]-attB parasites showed a slightly higher DIA sensitivity than NF54[Mito-roGFP2-Orp1]-attB parasites. CLSM data were composed of values from at least 10–20 trophozoites analyzed per experiment. Mean values and standard errors of the means (±SEM) are shown for three independent experiments. A one-way ANOVA test with 95% confidence intervals with the Dunnett’s Multiple Comparison Test was applied for statistical analysis of significance (***p < 0.001).

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