Figure 4

Mid- and long-term effects of ART, ATM, ATS, CQ, QN, MQ, AQ, and LUM on the redox ratio of P. falciparum NF54^{[roGFP2-Orp1]}-attB and NF54^{[Mito-roGFP2-Orp1]}-attB transfected parasites. 4 h incubation of NF54^{[roGFP2-Orp1]}-attB transfectants with CQ led to a significant increase of fluorescence ratio as detected using CLSM. (a) NF54^{[Mito-roGFP2-Orp1]} transfectants were significantly oxidized by ART, ATM, QN and MQ. (b) 24 h incubations oxidized both probes but did not lead to a significant increase of the 405/488 nm ratio. CLSM data were obtained from 10 to 20 trophozoites for each experiment and each incubation time. Mean values and standard errors of the means (±SEM) are shown for three independent experiments. A one-way ANOVA test with 95% confidence intervals with the Dunnett’s Multiple Comparison Test was applied for statistical analysis of significance (*p < 0.05; **p < 0.01; ***p < 0.001).