Figure 7 | Scientific Reports

Figure 7

From: TCA cycle rewiring fosters metabolic adaptation to oxygen restriction in skeletal muscle from rodents and humans

Figure 7The alternative text for this image may have been generated using AI.

(A) BCL2/adenovirus E1B 19 kDa protein-interacting protein 3 (Bnip3), Beclin-1 (Becn1), Apoptosis regulator Bcl-2 (Bcl2) and the lipidated form of microtubule-associated protein 1 light chain 3 beta (LC3B-II) immunoblotting and quantitation (cropped images; full lenght blots are included as Supplementary Data). (B) qRT-PCR of Becn1, apoptosis regulator Bax, Bcl2, Caspase-3 (Casp3), Bnip3, BCL2/adenovirus E1B 19 kDa interacting protein 3-like (Bnip3L), microtubule-associated protein 1 light chain 3 alpha (LC3A) and LC3B. Data were generated from eight independent experiments and values were referred to mRNA levels of control normoxic mice and expressed as mean ± SD. Immunoblotting and qRT-PCR data were subjected to a ANOVA and Tukey test (n = 5, p < 0.05). Differences between 2 H and 10 H versus N were indicated with the * symbol.

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