Figure 3 | Scientific Reports

Figure 3

From: Magnetic-Immuno-Loop-Mediated Isothermal Amplification Based on DNA Encapsulating Liposome for the Ultrasensitive Detection of P-glycoprotein

Figure 3

The real-time fluorescence curves of magnetic liposome-Im-LAMP assay reactions with different concentrations of P-gp:0 pg/ml (curve a), 160 pg/ml (curve b), 16 pg/ml (curve c), 1.6 pg/ml (curve d), 0.16 pg/ml (curve e), 1.6*10−2 pg/ml (curve f). (B) The relationship between cycle time and log concentrations of P-gp. The results are average values of three repetitive measurements. (C) Specificity of magnetic liposome-Im-LAMP assay detection of P-gp: the real-time fluorescence curves of Im-LAMP reactions with different reference proteins, bovine serum albumin (BSA), carcinoembryonic antigen (CEA), hemoglobin (HGB), immunoglobulin G (IgG) and the blank. The Im-LAMP reactions were performed in a volume of 10 µL. [FIP] = [BIP] = 1.6 µM, [B3] = [F3] = 0.2 µM, [Bst DNA polymerase] = 8 U/µL.

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