Figure 3

Analysis of tumour leucocytic infiltrate. Determination of immune subpopulations by flow cytometry, (a) T helper lymphocytes, (b) cytotoxic T lymphocytes, (c) macrophages and (e) Treg; *p = 0.0335 vs Intact, p = 0.0247 vs Vehicle. Data from 3 independent experiments are expressed as mean ± SD; n = 12. (d) Representative contour representations of cytometric analysis of Treg percentage; gate: CD3+, CD4+ from 10,000 lymphocytes collected.